Nanoscopy and Multidimensional Optical Fluorescence Microscopy

"Alberto Diaspro has been choreographing light’s dance for over 20 years, and in Nanoscopy and Multidimensional Optical Fluorescence Microscopy, he has assembled a diverse group of experts to explain the methods they use to coax light to reveal biology’s secrets."— From the Foreword by Daniel Evanko, editor, Nature Methods Nanoscopy and Multidimensional Optical Fluorescence Microscopy demonstrates that the boundaries between sciences do blur at the bottom, especially those that might separate the optical work of physicists and the cellular work of microbiologists. In 18 chapters written by pioneering researchers, this work offers the first comprehensive and current documentation of the cutting-edge research being accomplished in a wide range of photonic devices with revolutionary application. The highlight of the book is its coverage of optical nanoscopy and super-resolution microscopy. The rapid advances in this area over the past few years offer researchers in both photonics and molecular biologya wealth of accomplishment upon which they can build. Offering a complete treatment of this emerging field, this volume: Describes how scientists have exploited the properties of light and its fluorophore partners to overcome the resolution limit of conventional light microscopy Delves into recent ways to minimize the photobleaching that has long hampered many methods including those that have the potential to capture previously unobtainable information on the movements of single molecules Discusses the principles, benefits, and implementation of fluorescence correlation spectroscopy and related methods, which simplifies analysis by limiting light to stationary focal points in a sample Considers the most basic as well as emerging methods for improving three-dimensional optical sectioning microscopy Reviews the basics of FRET (fluorescence resonance energy transfer) and considers its new use for investigating protein complexes The text also introduces those emerging nonfluorescence microscopy methods that can actually exert mechanical forces to trap and move a variety of objects ranging from beads to living cells and cellular organelles. Combining this technique with fluorescence microscopy provides an unparalleled ability to manipulate and visualize biological samples. In the half-century since Richard Feynman challenged scientists to come up with the tools to investigate and manipulate our world at the nanoscale, we have succeeded in placing tools in the hands of biophysicists that are leading to major breakthroughs in our understanding of life and our ability to diagnose, treat, and prevent many challenges to human health. This book reflects what has been accomplished to date while pointing the way to what still needs to be done. Sted Microscopy With Compact Light Sources / Lars Kastrup ... [et Al.] -- Nonlinear Fluorescence Imaging By Saturated Excitation / Nicholas I. Smith ... [et Al.] -- Far-field Fluorescence Microscopy Of Cellular Structures At Molecular Optical Resolution / Christoph Cremer ... [et Al.] -- Fluorescence Microscopy With Extended Depth Of Field / Kai Wicker And Rainer Heintzmann -- Single Particle Tracking / Kevin Braeckmans ... [et Al.] -- Fluorescence Correlation Spectroscopy / Xianke Shi And Thorsten Wohland -- Two-photon Excitation Microscopy: A Superb Wizard For Fluorescence Imaging / Francesca Cella And Alberto Diaspro -- Photobleaching Minimization In Single- And Multi-photon Fluorescence Imaging / Partha Pratim Mondal ... [et Al.] -- Applications Of Second Harmonic Generation Imaging Microscopy / Paolo Bianchini And Alberto Diaspro -- Green Fluorescent Proteins As Intracellular Ph Indicators / Fabio Beltram ... [et Al.] -- Fluorescence Photoactivation Localization Microscopy / Manasa V. Gudheti, Travis J. Gould, And Samuel T. Hess -- Molecular Resolution Of Cellular Biochemistry And Physiology By Fret/flim / Fred S. Wouters And Gertrude Bunt -- Fret-based Determination Of Protein Complex Structure At Nanometer Length Scale In Living Cells / Valerică Raicu -- Automation In Multidimensional Fluorescence Microscopy: Novel Instrumentation And Applications In Biomedical Research / Mario Faretta -- Optical Manipulation, Photonic Devices, And Their Use In Microscopy / G. Cojoc ... [et Al.] -- Optical Tweezers Microscopy: Piconewton Forces In Cell And Molecular Biology / Francesco Difato ... [et Al.] -- In Vivo Spectroscopic Imaging Of Biological Membranes And Surface Imaging For High-throughput Screening / Jo L. Richens ... [et Al.] -- Near-field Optical Microscopy: Insight On The Nanometer-scale Organization Of The Cell Membrane / Davide Normanno, Thomas Van Zanten, And María F. García-parajo. Edited By Alberto Diaspro. A Chapman & Hall Book. Includes Bibliographical References And Index. STED microscopy with compact light sources / by Lars Kastrup ... [et al.] Nonlinear fluorescence imaging by saturated excitation / by Nicholas Smith, Katsumasa Fujita, Satoshi Kawata Far field fluorescence microscopy of cellular structures : molecular optical resolution / by Christoph Cremer ... [et al.] Fluorescence microscopy with extended depth of field / by Kai Wicker and Rainer Heintzmann Single particle tracking / by Kevin Braeckmans ... [et al.] Fluorescence correlation spectroscopy / by Xianke Shi and Thorsten Wohland Two photon excitation microscopy : a superb wizard for fluorescence imaging / by Cella Francesca and Alberto Diaspro Photobleaching minimization in single- and multi-photon fluorescence imaging / by Partha Pratim Mondal ... [et al.] Applications of second harmonic generation imaging microscopy / Paolo Bianchini and Alberto Diaspro Green fluorescent proteins as intracellular pH indicators / Ranieri Bizzarri ... [et al.] Fluorescence photoactivation localization microscopy / Manasa V. Gudheti, Travis J. Gould, and Samuel T. Hess Molecular resolution of cellular biochemistry and physiology by FRET/FLIM / Fred S. Wouters & Gertrude Bunt FRET-based determination of protein complex structure at nanometer length scale in living cells / by Valerica Raicu Automation in multidimensional fluorescence microscopy : novel instrumentation and applications in biomedical research / by Mario Faretta Optical manipulation, photonic devices, and their use in microscopy / by G. Cojoc ... [et al.] Optical tweezers microscopy : piconewton forces in cell and molecular biology / by Difato F. ... [et al.] Towards in vivo label-free, structural spectroscopic imaging of biological membranes and high-throughput screening using surface imaging techniques / by Richens, J.L. ... [et al.] Near-field optical microscopy : insight on the nanometer-scale organization of the cell membrane / by Davide Normanno, Thomas van Zanten, and Maria F. Garcia-Parajo. Contents......Page 5 Foreword......Page 7 Preface......Page 10 Editor......Page 12 Contributors......Page 13 1. STED Microscopy with Compact Light Sources......Page 17 2. Nonlinear Fluorescence Imaging by Saturated Excitation......Page 30 3. Far-Field Fluorescence Microscopy of Cellular Structures at Molecular Optical Resolution......Page 46 4. Fluorescence Microscopy with Extended Depth of Field......Page 81 5. Single Particle Tracking......Page 97 6. Fluorescence Correlation Spectroscopy......Page 114 7. Two-Photon Excitation Microscopy: A Superb Wizard for Fluorescence Imaging......Page 148 8. Photobleaching Minimization in Single- and Multi-Photon Fluorescence Imaging......Page 160 9. Applications of Second Harmonic Generation Imaging Microscopy......Page 188 10. Green Fluorescent Proteins as Intracellular pH Indicators......Page 202 11. Fluorescence Photoactivation Localization Microscopy......Page 224 12. Molecular Resolution of Cellular Biochemistry and Physiology by FRET/FLIM......Page 249 13. FRET-Based Determination of Protein Complex Structure at Nanometer Length Scale in Living Cells......Page 275 14. Automation in Multidimensional Fluorescence Microscopy: Novel Instrumentation and Applications in Biomedical Research......Page 293 15. Optical Manipulation, Photonic Devices, and Their Use in Microscopy......Page 314 16. Optical Tweezers Microscopy: Piconewton Forces in Cell and Molecular Biology......Page 344 17. In Vivo Spectroscopic Imaging of Biological Membranes and Surface Imaging for High-Throughput Screening......Page 363 18. Near-Field Optical Microscopy: Insight on the Nanometer-Scale Organization of the Cell Membrane......Page 376