Optical Nanoscopy and Novel Microscopy Techniques

Microscopy is at the forefront of multidisciplinary research. It was developed by physicists, made specific by chemists, and applied by biologists and doctors to better understand how the human body works. For this very reason, the field has been revolutionized in past decades.The objective of Optical Nanoscopy and Novel Microscopy Techniques is to choose some of those revolutionary ideas and serve a general audience from broad disciplines to achieve a fundamental understanding of these technologies and to better apply them in their daily research.The book begins with coverage of super-resolut. Read more... Front Cover; Contents; Preface; Contributors; chapter 1: Optical nanoscopy with stimulated emission depletion; chapter 2: Structured illumination microscopy; chapter 3: Super-resolution imaging with stochastic optical reconstruction microscopy (STORM) and photoactivated localization microscopy (PALM); chapter 4: Small-molecule labeling probes; chapter 5: Fluorescent proteins for optical microscopy; chapter 6: Single-molecule imaging with quantum dots; chapter 7: Fluorescence detection and lifetime imaging with stimulated emission; chapter 8: Fiber optic microscopy Chapter 9: Scanning ion conductance microscopychapter 10: Advanced photoacoustic microscopy; Back Cover Optical Nanoscopy with Stimulated Emission Depletion, Peng Xi, Hao Xie, Yujia Liu, and Yichen DingStructured Illumination Microscopy, Dan Dan, Baoli Yao, and Ming LeiSuper-Resolution Imaging with Stochastic Optical Reconstruction Microscopy (STORM) and Photoactivated Localization Microscopy (PALM), Yujie SunSmall-Molecule Labeling Probes, Jie Wang, Jie Li, Yi Yang, Maiyun Yang, and Peng R. ChenFluorescent Proteins for Optical Microscopy, Pingyong Xu, Mingshu Zhang, and Hao ChangSingle-Molecule Imaging with Quantum Dots, Mohammad U. Zahid and Andrew M. SmithFluorescence Detection and Lifetime Imaging with Stimulated Emission, Po-Yen Lin, Jianhong Ge, Cuifang Kuang, and Fu-Jen KaoFiber Optic Microscopy, Jin U. Kang and Xuan LiuScanning Ion Conductance Microscopy, Zhicong Fei, Hui Shi, Yanjun Zhang, and Yuchun GuAdvanced Photoacoustic Microscopy, Yichen Ding, QiushiRen, and Changhui LiIndex Front Cover 1 Contents 7 Preface 9 Contributors 11 chapter 1: Optical nanoscopy with stimulated emission depletion 15 chapter 2: Structured illumination microscopy 37 chapter 3: Super-­resolution imaging with stochastic optical reconstruction microscopy (STORM) and photoactivated localization microscopy (PALM) 75 chapter 4: Small-­molecule labeling probes 99 chapter 5: Fluorescent proteins for optical microscopy 125 chapter 6: Single-­molecule imaging with quantum dots 149 chapter 7: Fluorescence detection and lifetime imaging with stimulated emission 175 chapter 8: Fiber optic microscopy 193 chapter 9: Scanning ion conductance microscopy 213 chapter 10: Advanced photoacoustic microscopy 229 Back Cover 267 "Many new methods in light and super-resolution microscopy have been developed over the last few years. This edited volume overviews the latest advances in microscopy, complete with experimental methods and data analysis of the results. Each chapter begins with a brief description of the principles of the technique for clarity and concludes with a set of problems. Cutting-edge microscopy techniques presented include STED, PALM/STORM, and light sheet microscopy"--Provided by publisher