Multidimensional Microscopy

Modern cell biology is being revolutionized by the wedding of microscopy and computers. This book describes the new instrumentation and methods which allow three-dimensional reconstruction of specimens. Multidimensional Microscopy will be of interest to cell biologists, microscopists, and basic biomedical researchers whose work involves microscopic techniques. This book presents current results on a very active field in modern biology: methods in light and electron microscopy that allow the reconstruction of three-dimensional objects with the aid of computers. The book emphasizes the methods that can be used and examples of biological systems to which they have been applied. It includes extensive descriptions of confocal microscopy and its applications, as well as chapters on X-ray microscopy, low-voltage electron microscopy, and image reconstruction. This is an impressive summary of state-of-the-art methods in microscopy, in which microscopes and computers are being joined to permit specimens to be examined and reconstructed in three dimensions. Will be of interest to cell biologists, biomedical researchers, and microscopists. Front Matter....Pages i-xvii Confocal Microscopy: Basic Principles and System Performance....Pages 1-31 Designing a Confocal Fluorescence Microscope....Pages 33-51 Theory and Principal Technologies of the Laser Scanning Confocal Microscope....Pages 53-71 Simultaneous Ultraviolet and Visible Excitation Confocal Microscopy....Pages 73-85 High Resolution Confocal Microscopy of Phase and Amplitude Objects....Pages 87-102 Interferometric Microscopy — An Overview of the Optical Characterization of Inertial-fusion Targets....Pages 103-121 X-Ray Microscopy — Possibilities for Three Dimensional Imaging....Pages 123-132 High Resolution Projection X-Ray Microscopy....Pages 133-150 Cone-beam X-ray Microtomography....Pages 151-169 High Resolution Low Voltage Scanning Electron Microscopy: Reduced Radiation Damage on Cryo-specimens....Pages 171-190 Image Restoration in Light Microscopy....Pages 191-208 Image Quality Considerations in Computerized 2-D and 3-D Microscopy....Pages 209-230 Analysis and Presentation of Three Dimensional Data Sets....Pages 231-250 The Processing of Three-dimensional Confocal Data Sets....Pages 251-266 Effective Laser Lines for Biological Confocal Microscopy....Pages 267-289 Visualizing DNA Replication in Three Dimensions....Pages 291-303 Study of Nuclear Proteins Associated with Cell Proliferation, Differentiation, and Mitosis by Confocal Fluorescence Microscopy....Pages 305-319 Two- and Three-Dimensional Confocal Microscopy of the Eye....Pages 321-338 Confocal Microscopy of Botanical Specimens....Pages 339-380 Back Matter....Pages 381-387