Galectins: Methods And Protocols (methods In Molecular Biology)

__Galectins: Methods and Protocols__ is the first book solely dedicated to methodological approaches designed to study galectin function. The galectin family represents one of the most pleiotropic families, with individual members having been implicated in various aspects of nearly every biological process described, from RNA splicing to complex regulatory circuits that orchestrate adaptive immunity. Given the diverse roles of galectins in a variety of biological systems, studying these glycan binding proteins often requires the assimilation of diverse technical skills to fully appreciate their biological function. Their nearly ubiquitous expression and ability to bind highly modifiable carbohydrate ligands, in addition to a variety of other regulatory proteins, allows these glycan binding proteins (GBPs) to possess the capacity to regulate a wide variety of biological processes. Individual chapters are dedicated to examining salient features of galectin functions. Written in the successful __Methods in Molecular Biology__ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, __Galectins: Methods and Protocols__ seeks to serve both professionals and novices with a useful framework when examining galectin function for many years to come. Evolving Mechanistic Insights Into Galectin Functions / Connie M. Arthur ... [et Al.] -- Cloning, Expression, And Purification Of Galectins For In Vitro Studies / Paul A. Poland, Carol L. Kinlough, And Rebecca P. Hughey -- Alkylation Of Galectin-1 With Iodoacetamide And Mass Spectrometric Mapping Of The Sites Of Incorporation / Sean R. Stowell ... [et Al.] -- Evaluation Of Galectin Binding By Frontal Affinity Chromatography (fac) / Jun Iwaki And Jun Hirabayashi -- Probing Lectin-mucin Interactions By Isothermal Titration Microcalorimetry / Tarun K. Dam And C. Fred Brewer -- Examination Of Whole Cell Galectin Binding By Solid Phase And Flow Cytometric Analysis / Anne Leppänen, Connie M. Arthur, Sean R. Stowell, And Richard D. Cummings -- Evaluation Of Galectin Binding By Surface Plasmon Resonance / Padmaja Mehta-d'souza -- Examining Galectin Binding Specificity Using Glycan Microarrays / Connie M. Arthur ... [et Al.] -- Methods For Assessing The Effects Of Galectins On Leukocyte Trafficking / Beatrice R. Gittens, Rachael D. Wright, And Dianne Cooper -- Examination Of The Role Of Galectins In Plasma Cell Differentiation / Chih-ming Tsai And Kuo-i Lin -- Examination Of Galectin-induced Lattice Formation On Early B-cell Development / Stéphane J.c. Mancini ... [et Al.] -- Detection Of Phosphatidylserine Exposure On Leukocytes Following Treatment With Human Galectins / Connie M. Arthur ... [et Al.] -- Examination Of Galectins In Phagocytosis / Huan-yuan Chen ... [et Al.] -- Assessing The Roles Of Galectins In Regulating Dendritic Cell Migration Through Extracellular Matrix And Across Lymphatic Endothelial Cells / Sandra Thiemann, Jeanette H. Man, And Linda G. Baum -- Examination Of The Role Of Galectins In Intestinal Inflammation / Atsushi Nishida, Cindy W. Lau, And Atsushi Mizoguchi -- Study Of Galectins In Tumor Immunity : Strategies And Methods / Juan P. Cerliani ... [et Al.] -- Galectins In The Regulation Of Platelet Biology / Maria A. Romaniuk, Gabriel A. Rabinovich, And Mirta Schattner -- Examination Of The Role Of Galectins And Galectin Inhibitors In Endothelial Cell Biology / Iris A. E. Schulkens ... [et Al.] -- Regulation Of Galectins By Hypoxia And Their Relevance In Angiogenesis : Strategies And Methods / Mariana Salatino ... [et Al.] -- Examination Of The Role Of Galectins During In Vivo Angiogenesis Using The Chick Chorioallantoic Membrane Assay / Esther A. Kleibeuker ... [et Al.] -- Examination Of The Role Of Galectins In Cell Migration And Re-epithelialization Of Wounds / Zhiyi Cao ... [et Al.] -- Manipulating Galectin Expression In Zebrafish (danio Rerio) / Chiguang Feng ... [et Al.] -- Examination Of Galectin Localization Using Confocal Microscopy / Daniel Giuliano Cerri ... [et Al.] -- Examination Of The Regulation Of Galectin-3 Expression In Cancer / Hafiz Ahmed And Gargi Bandyopadhyaya -- Evaluation Of The Role Of Galectins In Parasite Immunity / Sarah Preston ... [et Al.] -- Effect Of Galectins On Viral Transmission / Michel Ouellet ... [et Al.] -- Evaluation Of The Bactericidal Activity Of Galectins / Connie M. Arthur, Richard D. Cummings, And Sean R. Stowell -- Examination Of The Role Of Galectins In Pre-mrna Splicing / Ronald J. Patterson ... [et Al.] -- Analysis Of The Intracellular Role Of Galectins In Cell Growth And Apoptosis / Daniel K. Hsu ... [et Al.] -- Nucleocytoplasmic Shuttling Of Galectin-3 / Eric J. Arnoys, Cheri M. Ackerman, And John L. Wang. Edited By Sean R. Stowell, Richard D. Cummings. Includes Bibliographical References And Index. Evolving mechanistic insights into galectin functions -- Connie M. Arthur [and three others] Cloning, expression, and purification of galectins for in vitro studies -- Paul A. Poland, Carol L. Kinlough, and Rebecca P. Hughey Alkylation of galectin-1 with iodoacetamide and mass spectrometric mapping of the sites of incorporation -- Sean R. Stowell [and three others] Evaluation of galectin binding by frontal affinity chromatography (FAC) -- Jun Iwaki and Jun Hirabayashi Probing lectin-mucin interactions by isothermal titration microcalorimetry -- Tarun K. Dam and C. Fred Brewer Examination of whole cell galectin binding by solid phase and flow cytometric analysis -- Anne Leppänen, Connie M. Arthur, Sean R. Stowell, and Richard D. Cummings Evaluation of galectin binding by surface plasmon resonance -- Padmaja Mehta-D'souza Examining galectin binding specificity using glycan microarrays -- Connie M. Arthur [and seven others] Methods for assessing the effects of galectins on leukocyte trafficking -- Beatrice R. Gittens, Rachael D. Wright, and Dianne Cooper Examination of the role of galectins in plasma cell differentiation -- Chih-Ming Tsai and Kuo-I Lin Examination of galectin-induced lattice formation on early B-cell development -- Stéphane J.C. Mancini [and five others] Detection of phosphatidylserine exposure on leukocytes following treatment with human galectins -- Connie M. Arthur [and five others] Examination of galectins in phagocytosis -- Huan-Yuan Chen [and four others] Assessing the roles of galectins in regulating dendritic cell migration through extracellular matrix and across lymphatic endothelial cells -- Sandra Thiemann, Jeanette H. Man, and Linda G. Baum Examination of the role of galectins in intestinal inflammation -- Atsushi Nishida, Cindy W. Lau, and Atsushi Mizoguchi Study of galectins in tumor immunity : strategies and methods -- Juan P. Cerliani [and seven others] Galectins in the regulation of platelet biology -- Maria A. Romaniuk, Gabriel A. Rabinovich, and Mirta Schattner Examination of the role of galectins and galectin inhibitors in endothelial cell biology -- Iris A. E. Schulkens [and four others] Regulation of galectins by hypoxia and their relevance in angiogenesis : strategies and methods -- Mariana Salatino [and ten others] Examination of the role of galectins during in vivo angiogenesis using the chick chorioallantoic membrane assay -- Esther A. Kleibeuker [and four others] Examination of the role of galectins in cell migration and re-epithelialization of wounds -- Zhiyi Cao [and three others] Manipulating galectin expression in Zebrafish (Danio rerio) -- Chiguang Feng [and seven others] Examination of galectin localization using confocal microscopy -- Daniel Giuliano Cerri [and six others] Examination of the regulation of galectin-3 expression in cancer -- Hafiz Ahmed and Gargi Bandyopadhyaya Evaluation of the role of galectins in parasite immunity -- Sarah Preston [and four others] Effect of galectins on viral transmission -- Michel Ouellet [and three others] Evaluation of the bactericidal activity of galectins -- Connie M. Arthur, Richard D. Cummings, and Sean R. Stowell Examination of the role of galectins in pre-mRNA splicing -- Ronald J. Patterson [and three others] Analysis of the intracellular role of galectins in cell growth and apoptosis -- Daniel K. Hsu [and three others] Nucleocytoplasmic shuttling of galectin-3 -- Eric J. Arnoys, Cheri M. Ackerman, and John L. Wang. Front Matter....Pages i-xv Back Matter....Pages 1-35 ....Pages 37-49