Fish Technology (springer Lab Manuals)

From the reviews: "Ever wondered how to catch your FISH ... in the dark pools of cellular biology? Then ... FISH technology might be a welcome addition to your library. Thirty-six chapters on 485 pages compile the latest protocols and background information on fluorescence in situ hybridization (or FISH) ... . Every chapter is illustrated by several schematic drawings ... . The manual is completed by a subject index ... . The book is certainly clear ... providing valuable protocols for both beginning FISHers and for FISH experts." (Dr. Jan Roelof Van der Meer, BioWorld, Issue 6, 2002) Front Matter....Pages I-X Front Matter....Pages 1-1 FISH Techniques, FISH Probes and Their Applications in Medicine and Biology — An Overview....Pages 3-50 Microscopy and Imaging Systems....Pages 51-69 Front Matter....Pages 71-71 FISH on Chromosome Preparations of Peripheral Blood....Pages 73-81 FISH on Uncultured Blood or Bone Marrow....Pages 82-89 FISH on Blood or Bone Marrow Smears....Pages 90-96 Epithelial Cells from Buccal Smears and Urine....Pages 97-108 FISH on Hair Root Cells....Pages 109-114 Amniotic Fluid Cells — Uncultured....Pages 115-126 Human Sperm Cells....Pages 127-137 Front Matter....Pages 139-139 Archival Cytogenetic Slides....Pages 141-147 Formalin-Fixed and Paraffin-Embedded Tissue Sections....Pages 148-161 Nucleus Extraction from Formalin Fixed/paraffin Embedded Tissue....Pages 162-170 Combined in Situ Nick Translation and mRNA FISH for Simultaneous Detection of Apoptosis and Expression of Apoptosis-Related Genes in Sections from Paraffin-Embedded Tissue....Pages 171-175 Nucleus Extraction from Cryofixed Tissue....Pages 176-184 Preparation of Samples for CGH by DOP-PCR from Cryofixed or Paraffin-Embedded, Microdissected Cells....Pages 185-196 Comparative Genomic Hybridization....Pages 197-217 Front Matter....Pages 219-219 High Resolution FISH of Stretched Chromosome Fibers....Pages 221-225 Quantitative DNA Fiber Mapping....Pages 226-253 PNA-FISH....Pages 254-261 Strand-Specific Fluorescence in Situ Hybridization: CO-FISH and COD-FISH....Pages 262-271 Front Matter....Pages 219-219 The Combined Use of Nuclease in Situ Digestion and FISH (NU-FISH)....Pages 272-281 DNA Breakage Detection-FISH (DBD-FISH)....Pages 282-290 Detection of Radiosensitivity by Means of Three-Color FISH....Pages 291-300 Fluorescence in Situ Hybridisation of a Complete Set of 41 Telomere Specific Probes in a Single Simultaneous Hybridisation Experiment Using the Chromoprobe Multiprobe System-T....Pages 301-306 Front Matter....Pages 307-307 Zoo-FISH....Pages 309-322 FISH on Insect Cells Transfected with Heterologous DNA....Pages 323-328 Yeast FISH: Delineation of Chromosomal Targets in Vegetative and Meiotic Yeast Cells....Pages 329-345 In Situ Detection of Viral Nucleic Acids by Chemiluminescence....Pages 346-359 Front Matter....Pages 361-361 Spectral Imaging Analysis of Metaphase and Interphase Cells....Pages 363-387 Microdissection of Chromosomes and Reverse FISH....Pages 388-407 Multicolor FISH Techniques for the Detection of Inter- and Intrachromosomal Rearrangements....Pages 408-424 Centromere-Specific Multicolor-FISH (cenM-FISH)....Pages 425-431 Simultaneous Multicolor-FISH and Immunocyto-chemical Analysis of Fresh Tumor Material....Pages 432-441 Simultaneous Fluorescence Immunophenotyping and FISH (FICTION)....Pages 442-460 Front Matter....Pages 461-461 PRINS: Hybridization and Primed in Situ Labeling in One Step....Pages 463-472 Microarrays and DNA-Chips in the Analysis of Genetic Aberrations....Pages 473-484 Back Matter....Pages 485-494 Fluorescence in situ hybridization (FISH) is a powerful method to directly visualise the localisation of genomic alterations in the nucleus. The technique has been adapted to a wide range of applications in both medicine, especially diagnostic cytogenetics, and biology. Topics described in this manual include: FISH on native human tissues, such as blood, bone marrow, epithelial cells, hair root cells, amniotic fluid cells, human sperm cells; FISH on archival human tissues, e.g. formalin-fixed and paraffin-embedded tissue sections, cryofixed tissue; simultaneous detection of apoptosis and expression of apoptosis-related genes; comparative genomic hybridization; special FISH techniques (Fiber-, PNA-, CO-, NU- and DBD-FISH); FISH on insect cells and yeast; multi-color FISH applications (spectral karyotyping analysis, reverse FISH and FICTION); FISH-related techniques, such as PRINS and microarrays Fluorescence in situ hybridization (FISH) has been developed as a powerful technology which allows direct visualisation or localisation of genomic alterations. The technique has been adopted to a range of applications in both medicine, especially in the areas of diagnostic cytogenetics, and biology. Topics described in this manual include: FISH on native human tissues, such as blood, bone marrow, epithelial cells, hair root cells, amniotic fluid cells, human sperm cells; FISH on archival human tissues, such as formalin fixed and paraffin embedded tissue sections, cryofixed tissue; simultaneous detection of apoptosis and xpression of apoptosis-related genes; comparative genomic ybridization; and special FISH techniques.