Enzyme Immunodiagnosis

Enzyme Immunodiagnosis focuses on the applications of enzyme immunodiagnosis procedures to the detection of drugs, infectious disease pathogens, and antigens in cancer pathology. This book discusses the use of immunoassays in vaccine assessment and standardization, including the application of commercial enzyme immunoassays (EIA) kits, automation of assays, and interpretation of results obtained with both serological and immunohistochemical methods. The developments in immunoblotting applications to diagnosis and research and use of polyclonal or monoclonal antibodies in enzyme immunodiagnosis are also reviewed. This book is beneficial to students and specialists conducting work on immunoenzymatic technology, as well as those involved in research, development, teaching, and diagnosis in fields in immunoassays. Content: Front Matter , Page iii Copyright , Page iv Preface , Pages ix-x 1 - Introduction , Pages 1-4 2 - Preparation of Immunoglobulins, Antibodies, or Fab Fragments , Pages 5-12 3 - Conjugation of Enzymes , Pages 13-22 4 - Principles of the Design of Enzyme Immunoassays , Pages 23-54 5 - Enzyme Immunohistochemical Methods , Pages 55-69 6 - Enzyme Immunoassays after Immunoblotting , Pages 70-76 7 - The Use of Polyclonal or Monoclonal Antibodies in Enzyme Immunoassays , Pages 77-85 8 - Homogeneous Enzyme Immunoassays for the Detection of Drugs , Pages 86-93 9 - The Value of Enzyme Immunohistochemistry in Pathology, Oncology, and Histology , Pages 94-115 10 - Enzyme Immunoassays for Infectious Diseases , Pages 116-171 11 - Automation in Enzyme Immunoassays and Commercial Kits , Pages 172-184 12 - Screening of Recombinant DNA Expression Libraries , Pages 185-191 13 - Vaccine Assessment and Standardization , Pages 192-195 14 - Future Prospects of Enzyme Immunodiagnosis , Pages 196-197 Acknowledgments , Page 198 Bibliography , Pages 199-221 Index , Pages 223-235