DNA Profiling and DNA Fingerprinting

This book reflects practical approaches to answering a variety of biological and medical questions using DNA fingerprinting and genetic profiling in a broad sense that also includes statistical evaluation of the data. It has been written for biology, biochemistry and medical students, graduates, postdoctoral fellows, academic teachers and for all researchers in the theoretical and applied sciences wherever genetic identification and relationship analysis are required. Front Matter....Pages I-X DNA Fingerprinting of Prokaryotic Genomes....Pages 1-15 Plant DNA Fingerprinting and Profiling....Pages 17-38 DNA Fingerprinting and Profiling in Behavioural Ecology....Pages 39-52 DNA Profiling in Veterinary Medicine....Pages 53-70 Multilocus DNA Fingerprinting....Pages 71-81 Various Levels of (Epi)Genetic Diversities as Demonstrable via Simple Repeated Sequences....Pages 83-99 Forensics: Analysis of Short Tandem Repeat Loci by Multiplex PCR and Real-Time Fluorescence Detection During Capillary Electrophoresis....Pages 101-115 Mitochondrial DNA: Diversity Analysis and Possible Pitfalls....Pages 117-131 PCR-based DNA Profiling of Human Y Chromosomes....Pages 133-152 The Use of Imperfect Microsatellites for DNA Fingerprinting and Population Genetics....Pages 153-165 PCR-SSCP: A Method for Reliably Detecting Single Nucleotide Polymorphisms in Multifactorial Diseases....Pages 167-176 Amplified Fragment Length Polymorphisms: A Non-Random PCR-Based Technique for Multilocus Sampling....Pages 177-194 Two-Dimensional DNA Fingerprinting....Pages 195-213 Statistical Approaches and Methods in Population Genetics Using Microsatellite Data....Pages 215-228 Statistical Inference from DNA Evidence....Pages 229-244 Back Matter....Pages 245-252 This manual presents practical approaches to using DNA fingerprinting and genetic profiling to answer a variety of biological and medical questions. It provides detailed methodology for setting up and performing experiments and evaluating results. Extensive troubleshooting tips, helpful hints, and advice for daily practice are also included. This will be a useful guide for scientists and researchers engaged in genetic identification and relationship analyses. DNA fingerprinting has become such an established technology that it has featured frequently in the media and is quite well known to the general public.