Chromatographic Methods

comprehensive and well presented...The chapters are informative and easy ot read...already used widely in teaching institutions and this use will undoubtedly continue in the future because it provides an ideal resource for this purpose. I commend it highly - Journal of Chromatography Since the inception of chromatography in 1903, the principal landmarks in its progress have been the virtual rediscovery of the technique in the 1930s, invention of synthetic resins in 1935, introduction of paper chromatography in the 1940s followed by that of thin layer, gas-solid and gas-liquid chroma- tography in the early 1950s. Whilst the theoretical aspects of HPLC were developed in the 1960s, it was the late 1970s before commercial instruments appeared. Developments through the 1980s in microelectronics and micro- processor technology afforded enhanced control, data acquisition and processing capabilities, and improved technologies for the manufacture of instrumentation. Developments in chromatographic media and packings and rapid scanning spectroscopic instruments have enabled combination techniques such as GC-MS, GC-IR, HPLC-MS and HPLC-IR to reach maturity and become standard routine techniques for the analyst. Further considerable research activity in the 1980s and early 1990s has led to the development of supercritical fluid chromatography (SFC), and high perfor- mance capillary electrophoresis is a technique that has proved invaluable in the genome project and the separation and typing of DNA fragments. Applications in environmental, health and safety, foods analysis and medical studies have contributed significantly to the advancement of these tech- niques. All of the instrumental chromatographic techniques are now used routinely by academic and industrial analysts. An understanding and experi- ence of such techniques is fundamental to the training of today's science undergraduates studying a range of disciplines reflecting the application areas mentioned above.

This book provides a unified and balanced introduction to the general theory of chromatography, followed by a detailed treatment of the principles and practice of all the major techniques currently employed in the industrial and academic sectors. It is written as a broad introduction to the subject for mid to advanced undergraduates in chemistry, pharmacy, biochemistry, and is suitable for students following the now quite numerous Masters degrees in instrumental analysis. The book has been updated to incorporate advances of the last ten years, and it contains around 50% new or revised material.

Front Matter....Pages i-xiv Introduction....Pages 1-16 Theoretical considerations....Pages 17-43 Planar chromatography....Pages 44-116 Liquid phase chromatography on open columns....Pages 117-164 Gas chromatography....Pages 165-257 High performance liquid chromatography....Pages 258-365 Chromatography and spectroscopic techniques....Pages 366-398 Processing chromatographic data....Pages 399-425 Model or practical experiments in chromatographic techniques....Pages 426-509 Illustrative problems in chromatography....Pages 510-524 Back Matter....Pages 525-559 Offers an introduction to the general theory of chromatography, and a treatment of the principles and practice of the major techniques employed in the industrial and academic sectors. This book is suitable for students following the numerous Masters degrees in instrumental analysis. This work presents an introduction to chromatography and reflects the significant developments since the mid-1980s, particularly in instrumentation and column technology, and control and data processing. The chapter on practical experiments has been revised for this edition