Basic Techniques in Molecular Biology (Springer Lab Manuals)

This laboratory manual gives a thorough introduction to modern, cost efficient techniques in Molecular Biology, which can find application in many different fields. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses and workshops or tested in the author's laboratory. Thus, they are also likely to work in inexperienced hands the first time they are performed. Step-by-step protocols and practical notes are provided. The distinguishing feature of this manual compared to others is the detailed explanation of the theoretical mechanisms of each step and the discussion of the importance of the techniques. In addition, each protocol includes an indication of the time and expense involved in its application. This knowledge will enable the users to design their own modifications or to adapt the method to different systems. Dr. Surzycki has been teaching undergraduate courses and leading workshops in introductory Molecular Biology for many years, during which time he has extensively modified and refined the techniques he has described in this manual.

This laboratory manual gives a thorough introduction to basic techniques in Molecular Biology, which can find application in many different fields. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. Thus, they are also likely to work in inexperienced hands the first time they are performed. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This knowledge will enable the users to design their own modifications or to adapt the method to different systems.
Surzycki has been teaching undergraduate courses and leading workshops for many years, during which time he has extensively modified and refined the techniques he has described in this manual.

This laboratory manual gives a thorough introduction to basic techniques in Molecular Biology, which can find application in many different fields. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the author's laboratory. Thus, they are also likely to work in inexperienced hands the first time they are performed. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This knowledge will enable the users to design their own modifications or to adapt the method to different systems. Surzycki has been teaching undergraduate courses and leading workshops for many years, during which time he has extensively modified and refined the techniques he has described in this manual Front Matter....Pages I-VIII General Aspects of DNA Isolation and Purification....Pages 1-32 Preparation of Genomic DNA from Animal Cells....Pages 33-56 Preparation of Genomic DNA from Plant Cells....Pages 57-78 Preparation of Genomic DNA from Bacteria....Pages 79-100 Isolation of Plasmid DNA....Pages 101-118 Isolation and Purification of RNA....Pages 119-144 Isolation of PolyA + RNA....Pages 145-162 Agarose Gel Electrophoresis of DNA....Pages 163-191 Preparation of Probes for Hybridization....Pages 192-220 Nucleic Acid Hybridization. A Theoretical Consideration....Pages 221-232 DNA Transfer and Hybridization....Pages 233-262 Northern Transfer and Hybridization....Pages 263-297 DNA Cloning. General Consideration....Pages 298-319 DNA Cloning - Experimental Procedures....Pages 320-373 DNA Sequencing....Pages 374-405 PCR Analysis....Pages 406-421 Back Matter....Pages 423-434 This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.